Anti -cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell
division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein
kinase, DKFZp686L20222)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IP IH IF |
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| Cdk1/p34cdc2 protein kinase is a catalytic subunit of Maturation Promoting Factor. p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. | | | Catalog # | C2549-50D | | Applications | Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Other applications have not been tested. | | Recommended Dilutions | Immunoprecipitation (Native, denatured): 2ug/mg protein lysate using Protein A. | | Immunohistochemistry (formalin/paraffin, frozen): 2-4ug/ml for 30 minutes at RT. Paraffin sections require antigen retrieval. | | Western Blot: 2ug/ml for 2 hours at RT. | | Optimal dilution determined by the researcher. | | Positive Controls | HeLa or NIH3T3 cells; germinal center in a tonsil or reactive lymph node. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 2Q305 | | Host | Mouse | | Source | Xenopus | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. | | Purity | Purified | | Immunogen | Xenopus p34cdc2. Cellular Localization: Nuclear with some cytoplasmic staining. | | Specificity | Recognizes Xenopus cdk1/p34cdc2 protein kinase at ~34kD. Does not crossreact with cdk2/p32 and other cyclin dependent kinases (cdks). Epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. Inhibits the activation of p43cdc2 kinase by cyclins and precipitates an active kinase. | | Species Crossreactivity | Human, mouse, rat, guinea pig, woodchuck, chicken. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein kinase, DKFZp686L20222) |
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