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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein kinase, DKFZp686L20222)

Anti -cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell
division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein
kinase, DKFZp686L20222)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH IF
Cdk1/p34cdc2 protein kinase is a catalytic subunit of Maturation Promoting Factor. p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues.
Catalog #C2549-50D
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation (Native, denatured): 2ug/mg protein lysate using Protein A.
Immunohistochemistry (formalin/paraffin, frozen): 2-4ug/ml for 30 minutes at RT. Paraffin sections require antigen retrieval.
Western Blot: 2ug/ml for 2 hours at RT.
Optimal dilution determined by the researcher.
Positive ControlsHeLa or NIH3T3 cells; germinal center in a tonsil or reactive lymph node.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q305
HostMouse
SourceXenopus
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified
ImmunogenXenopus p34cdc2. Cellular Localization: Nuclear with some cytoplasmic staining.
SpecificityRecognizes Xenopus cdk1/p34cdc2 protein kinase at ~34kD. Does not crossreact with cdk2/p32 and other cyclin dependent kinases (cdks). Epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. Inhibits the activation of p43cdc2 kinase by cyclins and precipitates an active kinase.
Species CrossreactivityHuman, mouse, rat, guinea pig, woodchuck, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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