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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -cdc2, Tyr15 (Cdk1, Cyclin Dependent Kinase 2)

Anti -cdc2, Tyr15 (Cdk1, Cyclin Dependent Kinase 2)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4).
Catalog #C2549-50T
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:10,000
Immunohistochemistry: 1:50
mmunocytochemistry: 1:500
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k113
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding Tyr15 of human Cdc2
SpecificityRecognizes human Cdc2 without phosphorylation on Tyrosine 15. Does not detect Y15-phosphorylated Cdc2. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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