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Anti -cdc2, Tyr15 (Cdk1, Cyclin Dependent Kinase 2)
Anti -cdc2, Tyr15 (Cdk1, Cyclin Dependent Kinase 2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC |
|
| During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4). | | | Catalog # | C2549-50T | | Applications | Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000-1:10,000 | | Immunohistochemistry: 1:50 | | mmunocytochemistry: 1:500 | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k113 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to residues surrounding Tyr15 of human Cdc2 | | Specificity | Recognizes human Cdc2 without phosphorylation on Tyrosine 15. Does not detect Y15-phosphorylated Cdc2. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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