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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -cdc27 (Cell Division Cycle 27)

Anti -cdc27 (Cell Division Cycle 27)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Catalog #C2550-11G
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:5,000-1:40,000
Western Blot: 1:500-1:2,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 0.02M potassium phosphate, pH 7.2, 0.15M sodium chloride, 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, NINDSLEIT, corresponding to aa422-430 of human cdc27.
SpecificityRecognizes human cdc27 at ~92kD. Species sequence homology: chimpanzee, chicken, rat and dog, 100%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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