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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -CDC27, NT (Cell Division Cycle Protein 27 Homolog, Anaphase-promoting Complex Subunit 3, APC3, CDC27 Homolog, CDC27Hs, H-NUC, ANAPC3, D0S1430E, D17S978E)

Anti -CDC27, NT (Cell Division Cycle Protein 27 Homolog, Anaphase-promoting Complex
Subunit 3, APC3, CDC27 Homolog, CDC27Hs, H-NUC, ANAPC3, D0S1430E, D17S978E)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Catalog #C2550-11H
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to Human CDC27 at N-terminal.
SpecificityRecognizes human CDC27.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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