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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -Cdc20 (Cdc20, Cdc20A, Cell division cycle 20, p55CDC, fizzy, bA276H19.3, MGC102824)

Anti -Cdc20 (Cdc20, Cdc20A, Cell division cycle 20, p55CDC, fizzy, bA276H19.3, MGC102824)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The anaphase-promoting complex (APC) or cyclosome is a cell cycle-regulated ubiquitin-protein ligase. It is composed of at least 11 subunits that controls exit from mitosis by degrading cyclins. It has been demonstrated that fizzy (also called Fzy or Cdc20) protein is essential for cyclosome activity. In Drosophila, fizzy is required for degradation of A and B type cyclins. Fizzy expression is cell cycle dependent with the peak occurring in M phase. Evidence suggests that fizzy is phosphorylated by Cdk1/cyclin B which inhibits its ability to activate the cyclosome in mitotic HeLa cells. Phosphorylation of fizzy may have a role in keeping the cyclosome inactive during early mitosis and under conditions of mitotic checkpoint arrest.
Catalog #C2559
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation (Native): 10ug/IP reaction
Western Blot: 1-5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa and NIH-3T3 cell lysates.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.125mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide before the addition of glycerol to 40%.
PurityPurified by epitope affinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminus of human zzy protein.
SpecificityRecognizes the zzy protein (~55kD). Species Crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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