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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -Cdc20 (Cdc20, Cdc20A, Cell division cycle 20, p55CDC, fizzy, bA276H19.3, MGC102824)

Anti -Cdc20 (Cdc20, Cdc20A, Cell division cycle 20, p55CDC, fizzy, bA276H19.3, MGC102824)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
The anaphase-promoting complex (APC) or cyclosome is a cell cycle-regulated ubiquitin-protein ligase. It is composed of at least 11 subunits that controls exit from mitosis by degrading cyclins. It has been demonstrated that fizzy (also called Fzy or Cdc20) protein is essential for cyclosome activity. In Drosophila, fizzy is required for degradation of A and B type cyclins. Fizzy expression is cell cycle dependent with the peak occurring in M phase. Evidence suggests that fizzy is phosphorylated by Cdk1/cyclin B which inhibits its ability to activate the cyclosome in mitotic HeLa cells. Phosphorylation of fizzy may have a role in keeping the cyclosome inactive during early mitosis and under conditions of mitotic checkpoint arrest.
Catalog #C2559-01B
Alternate NamesbA276H19.3, Cdc 20, Cdc20 cell division cycle 20 homolog, Cdc20A, Cell division cycle 20, Cell division cycle 20 homolog, Cell division cycle protein 20 homolog, fizzy, MGC102824, p55CDC
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:2000 Predicted molecular weight - 55kD
ELISA: 1:100-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8B71
HostMouse
SourceXenopus
Concentration~0.6mg/ml
FormSupplied as a liquid in PBS. No preservative added.
PurityPurified
ImmunogenSynthetic C-terminal peptide (Xenopus laevis); BLAST, FEVDPVTKKEKEKARSSKSIIHQSIR.
SpecificityRecognizes Xenopus laevis Cdc20.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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