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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -CDC25A, phosphorylated (Ser75) (M-phase Inducer Phosphatase 1, Dual Specificity Phosphatase Cdc25A)

Anti -CDC25A, phosphorylated (Ser75) (M-phase Inducer Phosphatase 1, Dual Specificity
Phosphatase Cdc25A)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Catalog #C2560-01G
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to amino acid residues surrounding Ser75 of human Cdc25A (KLH).
SpecificityRecognizes human Cdc25A phosphorylated at Ser75.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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