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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -cdc25A (Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, Dual specificity phosphatase CDC25A, M phase inducer phosphatase 1)

Anti -cdc25A (Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, Dual
specificity phosphatase CDC25A, M phase inducer phosphatase 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
Catalog #C2560-01P
ApplicationsSuitable for use in Western Blot andI Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation: 1-4 ul
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate buffer, pH 7-8, containing 0.09% sodium azide
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide within aa175-225 of human Cell Division Cycle 25a encoded by exon 7 (GeneID 993)
SpecificityRecognizes human cdc25A (exon 7)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCell division cycle 25A isoform a, Cell division cycle 25A isoform b, Dual specificity phosphatase CDC25A, M phase inducer phosphatase 1


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