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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CDCP1 (Cub Domain Containing Protein1, SIMA135)

Anti -CDCP1 (Cub Domain Containing Protein1, SIMA135)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
CDCP1 is a novel, 135kD cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa30-667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aas 270-300. This generates an 80kD TM protein that may be missing the N-terminal CUB domain (aa221-350). Over aas 25-667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.
Catalog #C2566-01
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1.0ug/ml. The detection limit of recombinant mouse CDCP1 is ~1ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit of recombinant mouse CDCP1 is ~25ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 5-15ug/ml detects CDCP1 in cells and tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa25-667 from the extracellular domain of mouse CDCP1 expressed in NSO cells (Q5U462).
SpecificityRecognizes mouse CDCP1. Shows ~25% crossreactivity with recombinant human CDCP1 in Direct ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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