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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CDCP1 (Cub Domain Containing Protein1, SIMA135)

Anti -CDCP1 (Cub Domain Containing Protein1, SIMA135)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
CDCP1 is a novel, 135kD cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa30-667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aas 270-300. This generates an 80kD TM protein that may be missing the N-terminal CUB domain (aa221-350). Over aas 25-667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.
Catalog #C2566-01
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml. Recombinant mouse CDCP1
Immunohistochemistry: 5-15ug/ml. Immersion fixed frozen sections of mouse embryo (E11-13)
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography
ImmunogenRecombinant protein corresponding to aa25-667 from mouse CDCP1, expressed in NSO cells (Q5U462).
SpecificityRecognizes mouse CDCP1. Shows ~25% crossreactivity with recombinant human CDCP1 in Direct ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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