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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CDCP1 (Cub Domain Containing Protein1, SIMA135)

Anti -CDCP1 (Cub Domain Containing Protein1, SIMA135)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
CUB domain containing protein 1 (CDCP1), also known as SIMA135, is a 140kD type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635aa extracellular region. The 148aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Catalog #C2566-01B
Recommended DilutionsDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~3ng/well.
Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~50ng/lane under non-reducing and reducing conditions. Chemiluminscent detection will increase sensitivity by 5 to 50 fold.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
Clone No9H98
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived, recombinant mouse CDCP1 (aa25-667) (Accession #Q5U462)
SpecificityRecognizes mouse CDCP1. Does not crossreact with recombinant human CDCP1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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