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Anti -CDCP1 (Cub Domain Containing Protein1, SIMA135)
Anti -CDCP1 (Cub Domain Containing Protein1, SIMA135)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
E B |
|
| CUB domain containing protein 1 (CDCP1), also known as SIMA135, is a 140kD type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635aa extracellular region. The 148aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein. | | | | | | Applications: | | | Suitable for use in Direct ELISA and Western Blot. Other applications not tested. | | | Catalog # | C2566-01B | | Recommended Dilutions | Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~3ng/well. | | Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~50ng/lane under non-reducing and reducing conditions. Chemiluminscent detection will increase sensitivity by 5 to 50 fold. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9H98 | | Host | Rat | | Source | Mouse | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0-derived, recombinant mouse CDCP1 (aa25-667) (Accession #Q5U462) | | Specificity | Recognizes mouse CDCP1. Does not crossreact with recombinant human CDCP1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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