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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -Cdk2, (Cyclin-dependent Kinase 2), phosphorylated (Thr14)

Anti -Cdk2, (Cyclin-dependent Kinase 2), phosphorylated (Thr14)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Cyclin-dependent protein kinase 2 (Cdk2), also known as cell division protein kinase 2, is a 34-kD nuclear protein that regulates events within G1 and S phase in the human cell cycle (1-4). Cdk2 is inactive until a complex is formed with cyclin A, D, or E (2). Inhibitory phosphorylation occurs on Thr14 and Tyr15. Activation of the Cdk2 complex requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK (3,4).
Catalog #C2572-08A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:5000-1:20,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:30
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E215
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.
SpecificityRecognizes human Cdk2 phosphorylated on Threonine 14 at ~33kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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