| CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (13). It consists of seven CEACAM (CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-specific glyco-protein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidy inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells. |
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| Catalog # | C2589-60 |
| Applications | Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunofluorescence: 50ug/ml Fixatition: 4% paraformaldehyde, permeabilized with 0.05% saponine. Diluted in PBS with 1% BSA |
| Immunohistochemistry: Frozen |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibody | IHC/IF |
| I1904-06F: IgG, H&L (X-Adsorbed) (FITC) Pab Gt xMo |
| I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5E502 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 40% glycerol. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (6). |
| Specificity | Recognizes human CEACAM1,3,4,5,6. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
