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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CEACAM 1,5,6,8 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)

Anti -CEACAM 1,5,6,8 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM (CEACAM1, CEACAM3- CEACAM8) and 11 pregnancy-
specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying
number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leukocytes. Over-expression
Catalog #C2589-62
ApplicationSuitable for use in flow cytometry, cell-ELISA, ELISA, Western blots and immunohistology on cryosections.
Specificity TestingBOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6; CEACAM7; CEACAM8; PSG1. An irrelevant monoclonal antibody served as a negative control. For specificity testing, protein G purified antibody was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM1, CEACAM5, CEACAM6 and CEACAM8 expressing cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No5E503
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by Protein G affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector.
ImmunogenImmunization of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).
SpecificityBroadly reactive antibody which reacts with most of the CEACAM molecules characterized to date (except CEACAM3, CEACAM4 and CEACAM7) (4). Can be used to detect CEACAM1 (BGP/CD66a), CEACAM5 (CEA/CD66e), CEACAM6 (NCA/CD66c) and CEACAM8 (CGM6/CD66b) on transiently transfected BOSC23 cells by flow cytometry. Included and characterized in the studies from the VIth Leucocyte Typing Workshop (4).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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