Anti -CEACAM 1,5,6,8 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH FC |
|
| CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM (CEACAM1, CEACAM3- CEACAM8) and 11 pregnancy- | | | specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying | | | number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leukocytes. Over-expression | | | Catalog # | C2589-62 | | Application | Suitable for use in flow cytometry, cell-ELISA, ELISA, Western blots and immunohistology on cryosections. | | Specificity Testing | BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6; CEACAM7; CEACAM8; PSG1. An irrelevant monoclonal antibody served as a negative control. For specificity testing, protein G purified antibody was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM1, CEACAM5, CEACAM6 and CEACAM8 expressing cells. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 5E503 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. | | Purity | Purified by Protein G affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector. | | Immunogen | Immunization of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3). | | Specificity | Broadly reactive antibody which reacts with most of the CEACAM molecules characterized to date (except CEACAM3, CEACAM4 and CEACAM7) (4). Can be used to detect CEACAM1 (BGP/CD66a), CEACAM5 (CEA/CD66e), CEACAM6 (NCA/CD66c) and CEACAM8 (CGM6/CD66b) on transiently transfected BOSC23 cells by flow cytometry. Included and characterized in the studies from the VIth Leucocyte Typing Workshop (4). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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