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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CEACAM 3 (Carcinoembryonic Antigen Related Cell Adhesion Molecule 3, CD66d, CGM1)

Anti -CEACAM 3 (Carcinoembryonic Antigen Related Cell Adhesion Molecule 3, CD66d, CGM1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Carcinoembryonic Antigen-related Cell Adhesion Molecule 3, (CEACAM3) also known as CD66d and CGM1, is a 35kD transmembrane glycoprotein in the CEACAM family of the immunoglobulin superfamily. The extracellular region consists of one Ig-like V-type domain, and the cytoplasmic tail contains one ITAM. CEACAM3 is upregulated on activated neutrophils and mediates their adhesion to vascular endothelial cells. CEACAM3 binds the Opa opacity proteins on some Gram negative bacteria, leading to bacterial uptake and neutrophil death. Alternate splicing generates an isoform with a substituted cytoplasmic tail and a potentially secreted isoform that contains a highly charged and Ser/Thr-rich sequence in place of the transmembrane and cytoplasmic regions. Orthologs of human CEACAM3 have not been identified in other species.
Catalog #C2589-68
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~3ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H213
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ull sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa35-155 from the extracellular domain of human CEACAM3 expressed in NSO cells (P40198).
SpecificityRecognizes human CEACAM3. Does not crossreact with recombinant human CEACAM1, CEACAM4, CEACAM5 or CEACAM6.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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