| CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy- |
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| specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the |
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| immunoglobulin (Ig) superfamily and are composed of one Ig variablelike (IgV) and a varying |
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| number (0-6) of Ig constantlike (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leukocytes. |
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| Catalog # | C2589-78 |
| Applications | MUS has been tested for the use in flow cytometry, cell-ELISA, ELISA, Western blots and immunohistology on cryosections. |
| Specificity testing of MUS | BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembraneanchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: BAP1. An irrelevant monoclonal antibody served as a negative control. For specificity testing, protein G purified MUS was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM5 and CEACAM6 expressing cells. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5E505 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. |
| Purity | Purified by Protein G affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector. |
| Immunogen | Immunization of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3). |
| Specificity | MUS reacts specifically with CEACAM5 (CEA/CD66e) and CEACAM6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
