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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CEACAM 5,6 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)

Anti -CEACAM 5,6 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-
specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the
immunoglobulin (Ig) superfamily and are composed of one Ig variablelike (IgV) and a varying
number (0-6) of Ig constantlike (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leukocytes.
Catalog #C2589-78
ApplicationsMUS has been tested for the use in flow cytometry, cell-ELISA, ELISA, Western blots and immunohistology on cryosections.
Specificity testing of MUSBOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembraneanchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: BAP1. An irrelevant monoclonal antibody served as a negative control. For specificity testing, protein G purified MUS was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM5 and CEACAM6 expressing cells.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5E505
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by Protein G affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector.
ImmunogenImmunization of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).
SpecificityMUS reacts specifically with CEACAM5 (CEA/CD66e) and CEACAM6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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