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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH IC

Cecropins are produced by insects, particularly under conditions of infection. Cecropins are bioactive peptides that exhibit activities by interacting with membranes and forming transmembrane channels that allow the free flow of electrolytes, metabolites and water across the phospholipid bilayers. Cecropins A, B and D are close homologues consisting of 35-39 residues. They are found in the pupae of the cecropin moth, but related homologues named lepodopteran, bactericidin, moricin and sarcotoxin are produced by other insects. Application Note(s): Anti-cecropin B is useful for the detection of cecropin B in various immunological assays. This reagent has specific value to study cecropins in transgenic plants. The antibody may also recognize naturally occurring cecropin A. Cecropin B has been modified in some instances by substituting a single amino acid to render the molecule less susceptible to proteolytic cleavage.
Catalog #	C2592-01
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000 (4400D)
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Concentration	~0.3mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetically produced full length cecropin B (Lys-Trp-Lys-Val-Phe-Lys- Lys-Ile-Glu-Lys-Val-Gly-Arg-Asn-Ile-Arg-Asn-Gly-Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala- Lys-Ala-Leu) corresponding to a modified sequence which is found in silkworm.
Specificity	Assay by IEP resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.