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You are here:Home » Biologicals-Cells, Plasma, Serum, Tissues » Cells-PBMC » Cells, PBMC, BioPrimary™

Cells, PBMC, BioPrimary™

Pricing

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Specifications

BioPrimary™ Cells are derived using technology including advanced protocols and adjuvants that ehance the viability of our cells to over >90% total post-thaw viability.
Catalog #C2600-160
A peripheral blood mononuclear cell (PBMC) is defined as any blood cell with a round nucleus (i.e. a lymphocyte, a monocyte, or a macrophage). These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells (~65%), B cells and Natural Killer cells (~28% combined), CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells (~7%).
These cells are often extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma. This buffy coat contains the PBMCs. Additionally, PBMC can be extracted from whole blood using a hypotonic lysis which will preferentially lyse red blood cells. This method results in neutrophils and other polymorphonuclear (PMN) cells which are important in innate immune defence being obtained.
All products are shipped on dry ice with 1) Certificate of Analysis (with viability and purity results), and 2) Thawing instructions.
Storage and Stability
It is recommended that cells are used as soon as possible following receipt to ensure optimal viability. Cells may be stored at -80 C for up to one month (minimize storage length at -80 C in order to maximize viability).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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