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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Cell Surface Marker (Neurons, Neuronal Precursors)

Anti -Cell Surface Marker (Neurons, Neuronal Precursors)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B
Because of their ability to identify neuronal precursors from a mixed culture of cells, antibodies to cell surface markers have become an important investigation tool in cell culture screening. Studies using the anti-Cell Surface Marker, clone mAb 2F7 have shown that this antibody labels the surfaces of neurons and neuronal precursors, but not mature oligodendrocytes and astrocytes derived from primary cultures (Schubert W, et al). Though the exact epitope of mAb 2F7 has not been determined, original characterization of this clone has revealed that the epitope is likely to be comprised of N-linked carbohydrates, and is localized on several high-molecular-weight membrane-associated proteins. Thus it is believed that anti-Cell Surface Marker, clone mAb 2F7 antibody recognizes a novel epitope region present on progenitor cells and postmitotic differentiating neurons in the developing mammalian nervous system.
Catalog #C2605-80
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlRat spinal cord E16 lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM,k
Clone No10H25
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenUntreated NT2/D1 cells corresponding to rat cell surface marker.
SpecificityRecognizes rat cell surfave marker. Species sequence homology: chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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