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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Cell Cycle Regulation BioAssay™ Sample Kit

Cell Cycle Regulation BioAssay™ Sample Kit

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Specifications

Activity of the cyclin-dependent kinases is regulated by T-loop phosphorylation, abundance of their cyclin partners and association with cdk inhibitors of the Cip/Kip or INK family of proteins. p27 Kip1 derives its ability to enforce the G1 restriction point from its inhibitory binding to cdk2/cyclin E and other cdk/cyclin complexes. In growing cells, extracellular mitogenic stimuli induces the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point in a pRbdependent entry into S-phase. The active complex of cyclin D/cdk4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1S-phase gene expression. In association with cdk2 complexes, p21 serves to inhibit kinase activity and block progression through G1/S, but it may also enhance assembly and activity in complexes of cdk4 or cdk6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression.
Catalog #C2612-10
Kit Components 1. p15 INK4B Pab Rb x 1x40ul 2. p16 INK4A Pab Rb x 1x 40ul 3. CDK4 Mab Mo x 1x40ul 4. CDK6 Mab Mo x 1x40ul 5. p27 Kip1 Pab Rb x 1x40ul 6. Cyclin D1 Mab Mo x 1x40ul 7. Cyclin D3 Mab Mo x 1x40ul 8. p21 Waf1/Cip1 Mab Mo x 1x40ul 9. IgG(HRP) Pab Gt x Rb 1x50ul 10. IgG(HRP) Pab Gt x Mo 1x100ul
PurityPurified by protein A and peptide affinity chromatography.
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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