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You are here:Home » Antibodies » Abs to Centromeres » Anti -CENP-A (Centromere Protein A, Histone H3-like Centromeric Protein A, Centromere Autoantigen A)

Anti -CENP-A (Centromere Protein A, Histone H3-like Centromeric Protein A, Centromere
Autoantigen A)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC FC
Centromere protein A (CENP-A) is a 17kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of -satellite DNA into primary chromatin structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.
Catalog #C2615-10A
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:80
Western Blot: 1:5000
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa Whole Cell lysate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H220
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to the C-terminal region of CENP-A, conjugated to KLH. Cellular Localization: Nucleus. Centromere. Kinetochore.
SpecificityRecognizes the C-terminal region of human CENP-A. Species Crossreactivity: rat. Does not react with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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