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Anti -CENP-A (Centromere Protein A, Histone H3-like Centromeric Protein A, Centromere Autoantigen A)
Anti -CENP-A (Centromere Protein A, Histone H3-like Centromeric Protein A, Centromere
Autoantigen A)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IH IC FC |
|
| Centromere protein A (CENP-A) is a 17kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of -satellite DNA into primary chromatin structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker. | | | Catalog # | C2615-10A | | Applications | Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:80 | | Western Blot: 1:5000 | | Immunohistochemistry: 1:250-1:500 | | Immunocytochemistry: 1:250-1:500 | | Optimal dilutions to be determined by the researcher. | | Positive Control | HeLa Whole Cell lysate. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H220 | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to the C-terminal region of CENP-A, conjugated to KLH. Cellular Localization: Nucleus. Centromere. Kinetochore. | | Specificity | Recognizes the C-terminal region of human CENP-A. Species Crossreactivity: rat. Does not react with mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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