| Centromere protein A (CENP-A) is a centromere-specific histone variant which forms an octameric complex with Histones H4, H2A, and H2B, in the presence of DNA. CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled. CENP-A is specifically located to the inner plate of the kinetochore and it is essential for kinetochore targeting of CENP-C and other kinetochore components. CENP-A is required for nucleosomal packaging of centromeric DNA at interphase. |
|
| Catalog # | C2615-24B |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry: 5ug/ml |
| Western Blot: 1ug/ml |
| Immunocytochemistry: 10ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 8J986 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 50% glycerol. No preservatives added. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide cooresponding to aa 3-19, PRRRSRKPEAPRRRSPS, of human CENP A. Cellular Localization: Nuclear. |
| Specificity | Recognizes human CENP A. at ~18kD. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | CENP A, CENPA, Centromere autoantigen A, Centromere protein A 17kDa, Histone H3 like centromeric protein A, centromere proteinAnti-centromeric protein A, CENP A, Centromere autoantigen A, Centromere protein A 17kDa, Histone H3 like centromeric protein A, |
