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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified E IH IC FC

Ceramides are a family of lipid molecules. A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in high concentrations within the cell membrane of cells. They are one of the component lipids that make up sphingomyelin, one of the major lipids in the lipid bilayer. For years, it was assumed that ceramides and other sphingolipids found in the bilayer cell membrane were purely structural elements. This is now known to be not completely true. Ceramide can actually act as a signaling molecule. The most well-known functions of ceramides as cellular signals include regulating the differentiation, proliferation, programmed cell death (PCD), and apoptosis (Type I PCD) of cells.
Catalog #	C2777-95
Applications	Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:10
	Flow Cytometry: 20ul/test
	Immunohistochemistry: 1:10
	Optimal dilutions to be determined by the researher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	6D311
Host	Mouse
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Purity	Purified by gel filtration on sephacryl S-300.
Immunogen	Ceramide (sphingosine-[trans-D-erythro-2-amino-4-octadecene-1,3-diol]), conjugated to BSA.
Specificity	Recognizes C16- and C24-ceramide, dihydroceramide, sphingomyelin and phosphatidylcholine in highly artificial lipid overlay test systems. Under more physiological in vitro conditions highly specific for ceramide and does not crossreact with sphingomyelin, cholesterol or other phospholipids.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.