| Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q (1). Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka- dependent signaling pathway (2); and indirectly enhances |
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| adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release (3). The active form of precerebellin is highly enriched in |
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| postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus (4). Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that |
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| precerebellin posseses functions other than those already stated (5). |
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| Catalog # | C2790-50 |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 2-4ug’ml |
| Immunohistochemistry: 2ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Mouse cerebellum cell lysate |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
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| Immunogen | Synthetic peptide consisting of 15aa from the middle of human |
| precerebellin (Genbank accession No. P23435). |
| Specificity | Recognizes human Precerebellin. Species Crossreactivity: mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
