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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Cfr13I (AsuI)

Cfr13I (AsuI)

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Specifications

Catalog #C3300
Sequence5'-G^G N C C-3'
3'-C C N G^G-5'
Restriction Enzyme Buffer A, 10X (R1625) Supplied to simplify buffer selection for double digests.
Enzyme PropertiesNumber of Recognition Sites in DNA:
Lambda: 74
PhiX174: 2
pBR322: 15
pUC57: 8
pUC18/19: 6
pTZ19R/U: 6
M13mp18/19: 4
Methylation Effects Cfr13I does not cut GGNCm5C. Blocked by overlapping Dcm and CG methylation.
Stability during Prolonged Incubation A minimum of 0.3units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal Inactivation Enzyme is inactivated by incubation at 65°C for 20min.
Compatible Ends CpoI, Eco47I, Psp5II, SanDI: G^G(A/T)CC
Quality ControlOverdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Cfr13I.
Ligation/Recutting Assay After 50-fold overdigestion (3u/ug DNA x 17 hours) with Cfr13I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.3uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6-12months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Citrobacter freundii RFL13
Concentration~10u/ul
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Storage Buffer (Form)Supplied as a liquid in 10mM potassium phosphate, pH 7.5 at 25°C, 100mM potassium chloride, 1mM EDTA, 7mM 2-mercaptoethanol, 0.2mg/ml BSA, 50% glycerol.
Diluent BufferFor short term storage (3-4weeks), use Restriction Enzyme Diluent Buffer, R1625-05: 10mM Tris-HCl (pH 7.4 at 25°C, 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. For long term storage, use the Storage Buffer should be used.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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