You are here:Home
»
Antibodies
»
Abs to Enzymes, Phosphodiesterase
»
Anti -cGMP Binding cGMP-Specific Phosphodiesterase V (PDE5)
Anti -cGMP Binding cGMP-Specific Phosphodiesterase V (PDE5)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| The superfamily of phosphodiesterases (PDE) catalyses the hydrolysis of 3':5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. PDE5 is a cGMP-specific enzyme that contains two allosteric cGMP binding sites in its amino-terminal regulatory domain (1). Elevated cGMP levels activates PKG, which phosphorylates PDE5 at serine 102 (2). Phosphorylation of PDE5 stimulates its enzymatic activity and enhances cGMP binding affinity in the regulatory domain, both lead to the decrease of intracellular cGMP levels (3,4). | | | Catalog # | C3400-01 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. | | Purity | Purified by Protein A and peptide immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to residues surrounding serine 102 of human PDE5 (KLH coupled). | | Specificity | Detects endogenous levels of total PDE5 (Mr 100kD). Does not crossreact with other phosphodiesterases. Species Crossreactivity: Human, monkey, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
