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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Channel Kinase 1 (CHAK1, EC 2.7.11.1, FLJ20117, FLJ25718, LTRPC7, TRP-PLIK, TRPM7, CHAK, Homolog of mouse transient receptor potential-phospholipase C-interacting kinase, LTRPC ion channel family member 7, Transient receptor potential cation channel subfa

Anti -Channel Kinase 1 (CHAK1, EC 2.7.11.1, FLJ20117, FLJ25718, LTRPC7, TRP-PLIK, TRPM7,
CHAK, Homolog of mouse transient receptor potential-phospholipase C-interacting kinase,
LTRPC ion channel family member 7, Transient receptor potential cation channel subfa

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role.
Catalog #C3445-26D
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml. If results are poor, use lysate without boiling, heat at 37ºC for 15 minutes
Immunocytochemistry (Perox): 0.1-1ug/ml
Immunocytochemistry (IF): 1-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F693
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenFusion protein corresponding to aa1817-1863 (cytoplasmic C-terminus) of mouse TrpM7 (Q923J1).
SpecificityRecognizes TrpM7. Does not react with TrpM6.
Species CrossreactivityHuman, mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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