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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10)

Anti -Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Homologs of E. coli Chaperonin 10 make up a family of small heat shock proteins with an approximate molecular mass of 10kD (Hsp10s). Chaperonin 10 acts as a co-chaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, Chaperonin 10 is thought to interact with cHsp60, a reported immunopathological antigen, identifying Chaperonine 10 as a potential contributor to an immunological response.
Catalog #C3470-01A
ApplicationsSuitable for use in RID, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionRID: 1:100,000
Western Blot: 1:2000-1:5000
Immunoprecipitation: 1:100
Immunohistochemistry: 1:400
Optimal dilutions to be determined by the researcher.
Positive ControlsH1838-01: HeLa Heat Shocked Cell Lysate
C3450: Recombinant Human Chaperonin 10 Protein
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein A chromatography.
ImmunogenSynthetic peptide corresponding to aa 92-102 RDGDILGKYVD of human Cpn10 (KLH). Cellular Localization: Mitochondria.
SpecificityRecognizes human Chaperonin 10 at ~10kD. Species Crossreactivity: mouse, rat, Xenopus laevis, bovine, canine, guinea pig, porcine, rabbit and sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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