| Homologs of E. coli Chaperonin 10 make up a family of small heat shock proteins with an approximate molecular mass of 10kD (Hsp10s). Chaperonin 10 acts as a co-chaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, Chaperonin 10 is thought to interact with cHsp60, a reported immunopathological antigen, identifying Chaperonine 10 as a potential contributor to an immunological response. |
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| Catalog # | C3470-01A |
| Applications | Suitable for use in RID, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | RID: 1:100,000 |
| Western Blot: 1:2000-1:5000 |
| Immunoprecipitation: 1:100 |
| Immunohistochemistry: 1:400 |
| Optimal dilutions to be determined by the researcher. |
| Positive Controls | H1838-01: HeLa Heat Shocked Cell Lysate |
| C3450: Recombinant Human Chaperonin 10 Protein |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. |
| Purity | Purified by Protein A chromatography. |
| Immunogen | Synthetic peptide corresponding to aa 92-102 RDGDILGKYVD of human Cpn10 (KLH). Cellular Localization: Mitochondria. |
| Specificity | Recognizes human Chaperonin 10 at ~10kD. Species Crossreactivity: mouse, rat, Xenopus laevis, bovine, canine, guinea pig, porcine, rabbit and sheep. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
