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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10) (Chlamydia)

Anti -Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10) (Chlamydia)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IC
Homologs of E. coli GroES make up a family of small heat shock proteins with an approximate molecular mass of 10kD (Hsp10s). Hsp10 acts as a cochaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, cpn10 is thought to interact with cHsp60, a reported immunopathological antigen (2), identifying cpn10 as a potential contributor to an immunological response (1).
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and ELISA. Other applications not tested.
Catalog #C3470A
Recommended DilutionWestern Blot: 0.25ug/ml
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No4E44
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.1mM PMSF, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenA 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on the C. trachomatis cpn10 (residues 54-69) (serovar A/HAR-13)
SpecificityDetects a 15kD protein, corresponding to the apparent molecular mass of cpn10 on SDS-PAGE immunoblots, in C. trachomatis infected cells. This antibody will not react with the human or mouse cpn10 homolog nor with E. coli GroES.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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