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You are here:Home » Biochemicals » Biochem-Biological Buffers » CHAPS ((3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate))

CHAPS ((3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate))

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Specifications

Non-denaturing zwitterionic detergent with cholic acid and sulfobetaine moieties, used to dissolve biological membranes, useful for protein extraction methods due to its ease of removal by dialysis or gel filtration. Low background UV absorption for protein detection.
Catalog #C3490
Designed by Good, et al., and typically referred to as Good’s buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes.
Melting Point270-272°C
SolubilityMethanol (Sparingly), Water
AtmosphereInert Gas
StabilityHygroscopic and Moisture Sensitive
Method for Determing IdentityH NMR Spectroscopic Analysis
TLC ConditionsSiO2; Methanol: NH4OH; 19: 1; Visualized with AMCS; Rf=0.3
CAS Number75621-03-3
Molecular FormulaC32H58N2O7S
Molecular Weight614.9
SourceSynthetic
Purity~90%
FormSupplied as a white to off white powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names(3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate)


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