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Anti -CHD1L (Chromodomain-helicase-DNA-binding Protein 1-like, Amplified in Liver Cancer Protein 1, ALC1)
Anti -CHD1L (Chromodomain-helicase-DNA-binding Protein 1-like, Amplified in Liver Cancer
Protein 1, ALC1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| CHD-1L is a 98-118kD member of the SNF2/RAD54 helicase family of proteins. It is expressed in hepatocytes, possesses ATPase activity, and likely promotes chromatin remodeling at sites of DNA damage. It also is considered an oncogene. CHD-1L upregulates ARHGEF9 trabscription, an action that promotes Cdc42 activity with accompanying filopodia formation and EMT. It also binds apoptosis-mediating Nur77, blocking its migration from nucleus-to-mitochondria. Human CHD-1L is 897aa in length. It contains a helicase ATP-binding domain aa58-223, a C-terminal helicase domain aa351-513, a coiled-coil region aa38-675 and one Macro domain that binds poly-ADP-ribose and targets DNA damage sites aa704-897. There are at least two Ser/Thr phosphorylation sites. There are multiple potential splice variants. One isoform contains an alternative start site at Met114, a second isoform possesses a deletion of aa43-246, a third isoform shows a three aa substitution for aa363-897, and a fourth isoform combines a 16aa insertion after 386aa with a five aa substitution for aa425-897. Over aa759-879, human CHD-1L shares 94% aa identity with mouse CHD-1L. | | | Catalog # | C3875-10J | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.1mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa759-879 from human CHD1L expressed in CHO cell line. | | Specificity | Recognizes human CHD1L. Species sequence homology: Recombinant human CHD1 and rhCHD5 (3%). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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