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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Chemerin, also known as TIG2, is a distant member of the cystatin superfamily. The chemerin precursor is cleaved by multiple serine proteases at various sites near the C-terminus. Mature active chemerin is a 15-16kD heparin binding protein that induces immature dendritic cell and macrophage chemotaxis through interactions with ChemR23. Mature human chemerin shares 65% and 70% aa sequence identity with mouse and rat chemerin, respectively.
Catalog #	C3890-01
Applications	Suitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended Dilutions	Western Blot: 1-2ug/ml. The detection limit for recombinant human Chemerin is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5 to 50 fold.
	Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Chemerin is ~3ng/well.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	7H141
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant protein corresponding to aa21-157 of human Chemerin expressed in E. coli (NP_002880).
Specificity	Recognizes human Chemerin. Does not crossreact with recombinant mouse Chemerin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.