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You are here:Home » Antibodies » Antibodies-Binding Proteins » Anti -Chemerin (TIG2)

Anti -Chemerin (TIG2)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Chemerin, also known as TIG2, is a distant member of the cystatin superfamily. The chemerin precursor is cleaved by multiple serine proteases at various sites near the C-terminus. Mature active chemerin is a 15-16kD heparin binding protein that induces immature dendritic cell and macrophage chemotaxis through interactions with ChemR23. Mature human chemerin shares 65% and 70% aa sequence identity with mouse and rat chemerin, respectively.
Catalog #C3890-01
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit for recombinant human Chemerin is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5 to 50 fold.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Chemerin is ~3ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H141
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa21-157 of human Chemerin expressed in E. coli (NP_002880).
SpecificityRecognizes human Chemerin. Does not crossreact with recombinant mouse Chemerin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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