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Chemerin, Recombinant, Human (Retinoic Acid Receptor Responder Protein 2, RAR-responsive Protein TIG2, Tazarotene-induced Gene 2 Protein, RARRES2, TIG2)
Chemerin, Recombinant, Human (Retinoic Acid Receptor Responder Protein 2, RAR-responsive Protein TIG2, Tazarotene-induced Gene 2 Protein, RARRES2, TIG2)
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| Chemerin is a chemoattractant expressed in white adipose, liver and lung tissues. Chemerin is a ligand for the G-protein coupled receptor known as ChemR23 (or chemokine-like receptor-1), which is expressed mainly on dendritic cells, macrophages and some adipocytes. Recombinant human Chemerin, produced in E. coli, is a non-glycosylated protein containing 138aa and having a total molecular mass of 16kD. | | | Catalog # | C3890-02 | | Source | Recombinant corresponding to human Chemerin, expressed in E. coli. | | AA Sequence | MELTEAQRRG LQVALEEFHK HPPVQWAFQE TSVESAAVDTP FPAGIFVRLE FKLQQTSRK RDWKKPECKV RPNGRKRKCL ACIKLGSEDK VLGRLVHCPI ETQVLREAEE HQETQCLRVQ RAGEDPHSFY FPGQFAFS | | Molecular Weight | ~16kD | | Endotoxin Level | <0.01ng/ug | | Biological Activity | Recombinant human Chemerin has full biological activity when compared standards. The ED50, as determined by its ability to chemoattract human Chem23R transfected BaF3 mouse pro-B cells, is typically 4-20ng/ml. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~16kD | | Source | E. coli | | Purity | ~98% (SDS-PAGE, HPLC) | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from 0.2% TFA. Reconstitute with sterile dH2O to 0.1mg/ml, which can be further diluted into other aqueous solutions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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