Anti -CHI3L1 (Chitinase-3-like Protein 1, 39kD Synovial Protein, Cartilage Glycoprotein
39, CGP-39, GP-39, hCGP-39, YKL-40)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E B IH |
|
| Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39kD glycoprotein member of the glycosyl hydrolase 18 family. CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. The mouse CHI3L1 cDNA encodes 381aa, including a 21aa signal sequence and a 360aa mature region with two intermolecular disulfides. Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin. CHI3L1 can bind heparins, probably as heparan sulfate. It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis. Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood. CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary. In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis. Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1. | | | Catalog # | C3980-01B | | Applications | Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:250-1:1500 | | Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b,k | | Clone No | AT4A3 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. | | Purity | Purified | | Immunogen | Recombinant corresponding to aa22-383 of human CHI3L1, expressed in E.coli. | | Specificity | Recognizes human CHI3L1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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