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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti -Chitinase 3-like 3 (Chitinase-3-like Protein 3, ECF-L, Eosinophil chemotactic cytokine, Secreted protein Ym1, Ym1 )

Anti -Chitinase 3-like 3 (Chitinase-3-like Protein 3, ECF-L, Eosinophil chemotactic
cytokine, Secreted protein Ym1, Ym1 )

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Mouse Chitinase 3-like 3 (CHI3L3), also known as ECF-L (eosinophil chemotactic factor-lymphocyte) or Ym1, is a secreted ~45kD glycoprotein that is a member of the glycosyl hydrolase family 18 (chitinase-like) protein family. Mouse CHI3L3 has no ortholog in humans, but shares 80% and 90% aa sequence identity with rat CHI3L3 and mouse CHI3L4 (also known as Ym2), respectively. Mouse CHI3L3 and CHI3L4 share partial overlap in cell type and tissue mRNA expression. CHI3L3 is primarily secreted by alveolar and peritoneal macrophages during inflammation, for example, due to nematode infection and airway hyper-responsiveness. It is considered a marker for alternatively activated macrophages, and is also expressed in bone marrow myeloid precursors, activated microglia, bone marrow-derived immature mast cells, connective tissue-type mast cells, macrophages in erythroblastic islands, neutrophil granules in bone marrow, peritoneum and spleen red pulp, and IL-4-stimulated B cells and dendritic cells (DC).
Catalog #C3980-01C
ApplicationsSuitable for use in ELISA and Western Blot Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse ECF-L is ~3ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse ECF-L is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7C3
HostRat
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa1-398 from mouse Chitinase 3-like 3 expressed in NSO cells (O35744).
SpecificityRecognizes mouse Chitinase 3-like 3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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