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You are here:Home » Culture Media » Media-Ingredients » Chick Embryo Extract, Ultrafiltrate (CEE)

Chick Embryo Extract, Ultrafiltrate (CEE)


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Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an ultrafiltration step with a 10kD MW cutoff to remove protein from the solution, producing a clear, amber liquid. This liquid is sterile-filtered.
Catalog #C3999
Animals are sourced from a specific USDA-Registered facility using a veterinary-inspected flock. Final processing at an ISO9000 facility. Chick Embryo Extract, Ultrafiltrate is protein free. It does not support the growth of viruses.
Appearance Pale-yellow, clear, solution
Total Protein (Biuret): ~0.0g/dL
Sterility (per 9CFR 113.26): Negative
Mycoplasma (per 9CFR 113.26): Negative
Osmolality: 260-360mOsm/Kg H2O
Microbial Testing:
Pullorum typhoid, Galisepticum, M. synoviae, M. meleagridis): Negative
Recommended Dilutions 1ml of extract/100ml of media
Storage and StabilityChick Embryo Extract, Ultrafiltrate is protein-free; thus, it will not break down. May be stored at RT. Stable for 12 months after receipt. Storage at 4°C extends the stability for an additional 3-6 months. Some precipitate has been observed when frozen.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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