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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti -Chitotriosidase (Chitinase 1, CHIT-1)

Anti -Chitotriosidase (Chitinase 1, CHIT-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Chitotriosidase, also known as chitinase 1, (CHIT1), is a 50kD secreted glycoprotein that belongs to glycohydrolase family # 18. It is synthesized by macrophages and serves to degrade chitin and chitotriose found in pathogens. Very high levels of CHIT1 are found in the plasma of patients with Gaucherís disease type I. The amino acid sequence of human CHIT1 is 99%, 76%, 75% and 55% identical to that of chimpanzee, rat, mouse and fruit fly.
Catalog #C4164-51
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG3
Clone No7H207
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived recombinant human CHIT1 (aa22-466) Accession # NP_003456
SpecificityRecognizes human Chitotriosidase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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