Chitotriosidase, also known as chitinase 1, (CHIT1), is a 50kD secreted glycoprotein that belongs to glycohydrolase family # 18. It is synthesized by macrophages and serves to degrade chitin and chitotriose found in pathogens. Very high levels of CHIT1 are found in the plasma of patients with Gaucher’s disease type I. The amino acid sequence of human CHIT1 is 99%, 76%, 75% and 55% identical to that of chimpanzee, rat, mouse and fruit fly.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Reconstitute with sterile PBS. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ala22-Asn466 from human CHIT1, expressed in NS0 cells.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Chitotriosidase. Does not cross-react with recombinant human CHI3-L1.