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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CHK1, phosphorylated (Ser317) (Serine/Threonine-protein Kinase Chk1, CHEK1)

Anti -CHK1, phosphorylated (Ser317) (Serine/Threonine-protein Kinase Chk1, CHEK1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Chk1, one of the downstream protein kinases of ATM/ATR, plays an important role in DNA damage checkpoint, embryonic development and tumor suppression. The activation of Chk1 in response to blocking DNA replication and certain forms of genotoxic stress involves phosphorylation of Ser317 and Ser345. Chk1 is also phosphorlaed at Ser280 and Ser296 following DNA damage. Activated Chk1 can inactivate cdc25c via phosphorylation at serine 216, blocking the activation of cdc2 and transition into M-phase. Chk1 can also phosphorylate p53 at serine 20 in vitro.
Catalog #C4200-05M
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA:1:4,000
Western Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human CHK1 around the phosphorylation site of serine 317 (S-S-SP-Q-P).
SpecificityRecognizes human Chk1 when phosphorylated at serine 317. Species Cross-Reactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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