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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CHK1 (CHEK1, Cell Cycle Checkpoint Kinase)

Anti -CHK1 (CHEK1, Cell Cycle Checkpoint Kinase)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IP
CHK1 has an important role at the G2 checkpoint in the cell cycle. CHK1 is activated in response to DNA damage, and serves to phosphorylate Cdc25, which results in cell cycle arrest.
Recent studies suggest that CHK1 may also play a role in the S phase checkpoint.
Catalog #C4200-15B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot 1:1,000-st1:10,000
Immunoprecipitation: 5-20ug/mg lysate
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsI1904-28C IgG, H&L (HRP) DKxGt
I1904-28G IgG, H&L (X-Adsorbed) (HRP) DKxGt
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~1mg/ml.
FormSupplied as a liquid in 0.09% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to a region within human CHK1.
SpecificityRecognizes human Cell Cycle Checkpoint Kinase Homologue 1 (CHK1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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