Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-Cancer Markers » Chk2, active, Recombinant, Human (Checkpoint Kinase 2, Rad 53)

Chk2, active, Recombinant, Human (Checkpoint Kinase 2, Rad 53)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Chk2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Serine 20 and p53 stabilization (2).
Catalog #C4201-15B
SourceRecombinant full-length human Chk2 was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_007194.
Molecular MassThe approximate molecular weight is 88kD.
ActivityThe specific activity of Chk2 was determined to be 729 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR).
StorageThis product is stable at -70°C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
SourceBaculovirus in Sf9 insect cells
Purity90% by densitometry
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links