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Specifications

Chlamydia trachomatis is propagated to maximum density in McCoy cells under Category 2 safety conditions. Infected cells are carefully washed and disrupted into buffered saline (PBS), subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris. Chlamydia antigen production is scalable from 10ml to several litres and is optimized by the multiplicity of infection and by harvesting time.
Catalog #	C4250-02C
	Inactivation is by heat treatment and the addition of triton X-100 to render products non-infectious and improve stability. All preparations are tested as no infectious by double passage on McCoy cell under optimal growth conditions.
Source	Chlamydia trachomatis, LGV2 strain 434, from clarified cell lysate.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Inactivation	Heat treatment and 0.05% Triton X-100. Tested inactive by two blind passages on permissive cells showing no signs of inclusion bodies.
Storage and Stability	Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note	No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.
Source	Clarified cell lysate
Purity	Purified
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.05% Triton X-100.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.