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You are here:Home » Molecular Biology » MB-Infectious Disease » Chlamydia trachomatis

Chlamydia trachomatis

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Specifications

Chlamydia trachomatis is propagated to maximum density in McCoy cells under Category 2 safety conditions. Infected cells are carefully washed and disrupted into buffered saline (PBS), subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris. Chlamydia antigen production is scalable from 10ml to several litres and is optimized by the multiplicity of infection and by harvesting time.
Catalog #C4250-02C
Inactivation is by heat treatment and the addition of triton X-100 to render products non-infectious and improve stability. All preparations are tested as no infectious by double passage on McCoy cell under optimal growth conditions.
SourceChlamydia trachomatis, LGV2 strain 434, from clarified cell lysate.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
InactivationHeat treatment and 0.05% Triton X-100. Tested inactive by two blind passages on permissive cells showing no signs of inclusion bodies.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NoteNo test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.
SourceClarified cell lysate
PurityPurified
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.05% Triton X-100.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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