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You are here:Home » Antibodies » Abs to Infectious Disease Chlamydia » Anti -Chlamydia LPS

Anti -Chlamydia LPS

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E
Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cellís nucleus. Chlamydia has a genome size of approximately 500-1000kB and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells, and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contain cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996).
-Kenneth Todar, University of Wisconsin-Madison
Catalog #C4250-51D
ApplicationsDirect ELISA
Recommended DilutionDirect ELISA: 1:10,000 dilution will yield 0.2 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig (against purified Chalmidia LPS)
ReconstitutionReconstitute with 0.5ml of dH2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No5C34
HostMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2.
PurityPurified by ion exchange chromatography.
ImmunogenPurified Chlamidia LPS
SpecificityRecognizes Chlamydia LPS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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