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You are here:Home » Antibodies » Abs to Infectious Disease Chlamydia » Anti -Chlamydia LPS

Anti -Chlamydia LPS

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH IF
LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella Minnesota or Neisseria gonorrhoeae.
Catalog #C4250-51F
ApplicationsSuitable for use in Immunofluorescence Microscopy, Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionsImmunofluorescence Microscopy: 1:10 with PBS, pH 7.4.
Immunohistochemistry: Paraformaldehyde-fixed tissue sections. Detects chlamydia species in samples after fixation with methanol/acetone (1:1).
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C. Stable for 12 months after receipt at 4°C. Reconstitute with sterile ddH2O. Store at 4°C. Reconstituted product is stable for 12 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. DO NOT FREEZE! IgG3 isotype antibodies tend to form aggregates upon thawing.
Clone TypeMonoclonal
IsotypeIgG3
Clone No5F153
HostMouse
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenChlamydia antigen
SpecificityRecognizes a genus-specific epitope of the Chlamydia lipopolysaccharide antigen and identifies 15 serotypes of C. trachomatis as well as C. psittaci and C. pneumoniae with a strong fluorescence of the intracellular inclusions, the pinhead-sized extracellular elementary bodies and the free cell-associated Chlamydia lipopolysaccharide antigens (amorphous foci).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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