USBio Logo

C4250-51F Mouse Anti-Chlamydia LPS

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG3
Clone Number
5F153 (ACI)
Grade
Affinity Purified
Applications
E IF IHC
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella Minnesota or Neisseria gonorrhoeae.

Applications
Suitable for use in Immunofluorescence Microscopy, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Immunofluorescence Microscopy: 1:10 with PBS, pH 7.4. Immunohistochemistry: Paraformaldehyde-fixed tissue sections. Detects chlamydia species in samples after fixation with methanol/acetone (1:1). Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder and reconstituted products must be stored at 4°C. Reconstitute with sterile ddH2O. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Chlamydia antigen
Form
Supplied as a lyophilized powder from PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes a genus-specific epitope of the Chlamydia lipopolysaccharide antigen and identifies 15 serotypes of C. trachomatis as well as C. psittaci and C. pneumoniae with a strong fluorescence of the intracellular inclusions, the pinhead-sized extracellular elementary bodies and the free cell-associated Chlamydia lipopolysaccharide antigens (amorphous foci). Also reacts with C. pecorum.
References
1. Näher, H., Petzoldt, D., Sethi, K.K. Evaluation of non-radioactive in situ hybridisation method to detect Chlamydia trachomatis in cell culture. In: Genitourin Med. 64: 162-164 (1988).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved