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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH IF

LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella Minnesota or Neisseria gonorrhoeae.
Catalog #	C4250-51F
Applications	Suitable for use in Immunofluorescence Microscopy, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions	Immunofluorescence Microscopy: 1:10 with PBS, pH 7.4.
	Immunohistochemistry (Paraffin): Paraformaldehyde-fixed tissue sections.
	Immunohistochemistry (Frozen): Detects chlamydia species in samples after fixation with methanol/acetone (1:1).
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG3
Clone No	5F153
Host	Mouse
Concentration	~0.05mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Chlamydia antigen
Specificity	Recognizes a genus-specific epitope of the Chlamydia lipopolysaccharide antigen and identifies 15 serotypes of C. trachomatis as well as C. psittaci and C. pneumoniae with a strong fluorescence of the intracellular inclusions, the pinhead-sized extracellular elementary bodies and the free cell-associated Chlamydia lipopolysaccharide antigens (amorphous foci).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.