| Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells, and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contain cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996). Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell’s nucleus. Chlamydia has a genome size of ~500-1000kb and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained. |
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| -Kenneth Todar, University of Wisconsin-Madison |
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| Catalog # | C4250-69 |
| Applications | Suitable for ELISA. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by researcher. |
| Source | Ascites |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Also Available | C4250-60: Chlamydia trachomatis MOMP (Major Outer Membrane Protein) Pab Gt x |
| C4250-60A: Chlamydia trachomatis MOMP (Major Outer Membrane Protein) (Azide Free) Pab Gt x |
| C4250-66: Chlamydia trachomatis MOMP (Major Outer Membrane Protein) (Biotin) Pab Gt x |
| C4250-67: Chlamydia trachomatis MOMP (Major Outer Membrane Protein) (FITC) Pab Gt x |
| C4250-69: Chlamydia trachomatis MOMP (Major Outer Membrane Protein) (HRP) Pab Gt x |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Concentration | ~1-3mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, PBS, 10 mg/ml BSA, 0.002% Thimerosal. Labeled with horseradish peroxidase (HRP). Estimated Molar HRP:IgG substitution is 2-3. |
| Purity | Purified by sodium sufate precipitation and ion-exchange chromatography. |
| Immunogen | Chlamydia Trachomatis strain L2, purified Major Outer Membrane Protein (MOMP). |
| Specificity | Recognizes Major Outer Membrane Protein (MOMP). Recognizes all serovars (A-K, L1-L3). Negative vs HEp-2 cells and egg yolk sac Does not crossreact with C. psittacii or C. pneumoniae in MIF. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
