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Anti -Chlamydia trachomatis MOMP (Major Outer Membrane Protein) (FITC)
Anti -Chlamydia trachomatis MOMP (Major Outer Membrane Protein) (FITC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Highly Purified |
IF |
|
| Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell’s nucleus. Chlamydia has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contains cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival. | | | Catalog # | C4250-70E | | Applications | Suitable for use in Immunofluorescence. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. | | Optimal dilutions to be determined by the researcher. | | End-Point Titer | >1:1000 by direct IF vs. all serovars (A-K, L1-L3) | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Concentration | ~4-5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, BSA (10mg/ml), 0.1% sodium azide. Conjugated to FITC. | | Purity | Purified IgG fraction to a purity of > 95%. | | Immunogen | Purified Major Outer Membrane Protein (MOMP) from Chlamydia trachomatis strain L2 | | Specificity | Recognizes Major Outer Membrane Protein (MOMP). Does not react with C. psittacii or C. pneumoniae in MIF. Uninfected Cell Reactivity: Negative vs. HEp-2 cells and egg yolk sac. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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