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You are here:Home » Antibodies » Abs to Infectious Disease Chlamydia » Anti -Chlamydia trachomatis, Outer Membrane Complex Protein

Anti -Chlamydia trachomatis, Outer Membrane Complex Protein

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IF
Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cellís nucleus. Chlamydia has a genome size of ~500-1000KB and contains both RNA and DNA. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells,and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K) and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contains cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival.
Catalog #C4250-85
ApplicationsSuitable for use in Immunofluorescence Microscopy and Detection. Other applications not tested.
Recommended DilutionsImmunofluorescence Microscopy (Cell culture, frozen): 1:10 diluted with PBS, pH 7.4.
Detection: Detects chlamydia species in clinical specimens and cell culture
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F122
HostMouse
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenChlamydia antigen
SpecificityRecognizes the 60kD outer membrane complex protein present in the periphery of reticulate bodies (RBs) of 15 serotypes of Chlamydia trachomatis and Chlamydia psittaci. The epitope is also present on elementary bodies (EBs) of C. trachomatis C-complex serotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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