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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B

The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
Catalog #	C4275-35C
Applications	Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:20-1:200
	ELISA: 1:200-1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.002% Thimerosal. Labeled with HRP.
Purity	Purified
Immunogen	Cholera Toxin, beta epitope (purified choleragenoid).
Specificity	Recognizes the beta subunit of Cholera toxin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.