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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E IH IF

Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
Catalog #	C4275-35P
Applications	Suitable for use in ELISA, Immunofluorescence, and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: >1:1,000
	Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~4-5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with FITC.
Purity	Purified IgG fraction to a purity of > 95%.
Immunogen	Purified Choleragenoid
Specificity	Recognizes Beta subunit of Cholera toxin. Immunoprecipitates toxin in gel techniques.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.