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You are here:Home » Antibodies » Abs to Infectious Disease Cholera » Anti -Cholera Toxin, beta (Cholera Enterotoxin beta, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456) (HRP)

Anti -Cholera Toxin, beta (Cholera Enterotoxin beta, Choleragenoid, CTX B, CTXB, CTX-B,
TOXB, VC1456) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IP IC Gel
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
Catalog #C4275-35Q
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry, and Immunoprecipitation (Gel). Other applications not tested.
Recommended DilutionImmunocytochemistry: 1:20-1:200
Western Blot: 1:20-1:200
ELISA: 1:200-1:000
Optimal dilutions to be determined by the researcher.
End-Point Titer >1:1000 by ELISA
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1-2mg/ml
FormSupplied as a liquid in PBS, 10mg/ml BSA, 0.002% thimerosal. Labeled with HRP. Estimated molar HRP
PurityPurified IgG fraction to a purity of ~95%.
ImmunogenPurified Choleragenoid.
SpecificityRecognizes the beta subunit of Cholera toxin. Immunoprecipitates toxin in gel techniques.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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