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You are here:Home » Antibodies » Abs to Infectious Disease Cholera » Anti -Cholera Toxin

Anti -Cholera Toxin

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
Catalog #C4275-39
ApplicationsSuitable for use in ELISA. Other applications have not been tested.
Recommended DilutionELISA: 1:10e-3
Optimal dilutions to be determined by the researcher.
Hybridoma:
Hybridization of SP2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.B.203
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide and 40% glycerol.
Purity>90%. Purified by Protein A Sepharose chromatography. Purity is tested by electrophoresis.
ImmunogenPurified beta-subunit of cholera toxin.
SpecificityBeta-subunit of chorera toxin. Reacts with cholera toxin and enterotoxin of E. coli.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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