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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
Catalog #	C4275-39J
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	ELISA: 1:20-1:200
	Optimal dilutions to be determined by the researcher.
Matched Pairs (capture/detection)	C4275-39H / C4275-39H
	C4275-39J / C4275-39H
	C4275-39J / C4275-39K
	Storage and Stability:
	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8D122
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 0.01M PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Cholera toxin
Specificity	Recognizes the B subunit of cholera toxin. Reacts with intact toxin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.