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You are here:Home » Antibodies » Abs to Infectious Disease Cholera » Anti -Cholera Toxin

Anti -Cholera Toxin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.
Catalog #C4275-39J
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 1:20-1:200
Optimal dilutions to be determined by the researcher.
Matched Pairs (capture/detection)C4275-39H / C4275-39H
C4275-39J / C4275-39H
C4275-39J / C4275-39K
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8D122
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in 0.01M PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenCholera toxin
SpecificityRecognizes the B subunit of cholera toxin. Reacts with intact toxin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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